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The capacity of bacteria to replicate the same DNA sequence at the same time and place has made them great model organisms to study the nucleic acid sequence. In some bacteria, one particular DNA sequence within a chromosome may be replicated rapidly. The other sequences, referred to as a lagging strand or a second strand, are synthesized at a slower rate.
For example, in E. coli, DNA replicates using the “sliding clamp” method. The lagging strand is replicated by synthesizing a new DNA strand on the surface of a protein loop that encircles the parent strand. The process begins with the replication of a DNA strand in the center of the loop. Once the lagging strand is synthesized, the loop slides to the right and completes the replication of the entire genome. While the replication of the lagging strand is still underway, a protein loader synthesizes a new lagging strand that is complementary to the original lagging strand. The result is the creation of two identical copies of the parent DNA strand.
The ability to identify the positions of genes allows the sequence of the entire genome to be broken down into a specific set of ordered sequences, which can be used to identify regulatory sequences and information about a given organism.
As the exponential advancement of molecular biology has brought a remarkable revolution in the study of nucleic acids, it has also made the genomic sequence of many organisms available.
Gene identification in high-resolution genome projects has made it possible to obtain the sequences of not only a few genomes but also several other genomes for the same species.
With the exponential growth of genomic data, there is an increasing need to perform genome-wide analysis. However, the complexity of existing genome sequences has prevented the identification of most genes in a genome. For a genome to be used in a genome-wide analysis, the location of genes must be accurately identified. A significant bottleneck exists between gene isolation and gene-docking.
The step of isolating the desired gene, typically by PCR, is very important since the gene
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